Lactoperoxidase-catalyzed Iodination of Membrane Proteins in Normal and Neoplastic Epidermal Cells
نویسندگان
چکیده
منابع مشابه
Accessibility of proteins in rat liver-free and membrane-bound ribosomes to lactoperoxidase-catalyzed iodination.
We have used lactoperoxidase-catalyzed iodination to study the topographical distribution of proteins in free and membrane-bound polysomes and in isolated ribosomal subunits. Free polysomes or rough microsomes were iodinated with 125I and then dissociated into 40 S and 60 S subunits by the use of puromycin/KCl. The separated subunits were iodinated with 13’1 and their protein composition analyz...
متن کاملLactoperoxidase-catalyzed iodination of horse cytochrome c:monoiodotyrosyl 74 cytochrome c.
Iodination of horse cytochrome c with the lactoperoxidase-hydrogen peroxide-iodide system results initially in the formation of the monoiodotyrosyl 74 derivative. This singly modified protein was obtained in pure form by ion exchange chromatography and preparative column electrophoresis. It shows an intact 695 nm absorption band, the midpoint potential of the native protein, a nuclear magnetic ...
متن کاملThe transmembrane proteins in the plasma membrane of normal human erythrocytes. Evaluation employing lactoperoxidase and proteases.
The molecular architecture of the human erythrocyte membrane has been probed using lactoperoxidase-catalyzed iodination in conjunction with Pronase hydrolysis. Resealed, hemoglobin-free ghosts were labeled at the cytoplasmic surface and the external membrane surface was subsequently digested with Pronase. Changes in size of the components labeled at the cytoplasmic surface were readily detected...
متن کاملlactoperoxidase-reactive surface membrane proteins in nonneoplastic erythmoid cells during cell maturation and in neoplastic erythnoid cells as compared with nonneoplastic enythroid cells at similar stages of development
The developmental pattern of one class of plasma mem brane proteins, the external surface proteins, was examined in neoplastic and nonneoplastic differentiating munine emythroid cells. Neoplastic enythroid precursor cells were obtained from spleens of CD-i mice after infection with Friend emythroleukemia virus while the nonneoplastic cells were obtained from spleens of mice with phenylhydrazine...
متن کاملNormal and Neoplastic Stem Cells
A stem cell is broadly defined as a cell that retains the capacity to self-renew, a feature that confers the ability to continuously make identical daughter cells or additional cells that will differentiate into downstream progeny. This highly regulated genetic program to retain "stemness" is under active investigation. Research in our laboratory has explored similarities and differences in emb...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Investigative Dermatology
سال: 1982
ISSN: 0022-202X
DOI: 10.1111/1523-1747.ep12497859